47614
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47614
Fluka
Fluram®
BioChemika, for fluorescence, ≥99.0% (UV)
| Synonym: | 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione, Fluorescamine |
| CAS Number: | 38183-12-9 |
| Linear Formula: | C17H10O4 |
| Molecular Weight: | 278.26 |
| Beilstein Registry Number: | 921143 |
| EC Number: | 253-814-5 |
| MDL number: | MFCD00005928 |
| PubChem Substance ID: | 24871213 |
Description
| Application | Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds.1,2 Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes.3 Effectively blocks newly generated amino termini in protein sequence analyses. |
| Legal Information | ® Registered Trademark of Hoffmann-La Roche & Co. AG |
| Fluram is a registered trademark of Hoffman-LaRoche & Co. AG |
Properties
| grade | for fluorescence |
| product line | BioChemika |
| assay | ≥99.0% (UV) |
| fluorescence | λex 390 nm; λem 475 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine) |
| mp | 153-157 °C(lit.) |
| 153-157 °C | |
| solubility | acetonitrile: soluble |
| ethanol: soluble |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| Cited Reference | 1. Lai, C.Y. Meth. Enzymol. 47, 236, (1977) |
| 2. Udenfriend, S. Science 178, 871, (1972) | |
| 3. Sogowa, K., and Takahaski, K., Use of fluorescamine-labeled casein as a substrate for assay of proteinases. J. Biochem. Tokyo 83, 1783, (1978) | |
| reference | Stein, S., et al., Amino acid analysis with fluorescamine at the picomole level. Arch. Biochem. Biophys. 155, 202, (1973) |
| Bhown, A.S., et al. Anal. Biochem. 112, 158, (1981) | |
| Merck | Merck 13,4184 |
| FT-IR 1 (2), 1009:D / FT-IR 2 (3), 4139:B / FT-NMR 1 (3), 554:C / IR-Spectra (3), 1472:H / RegBook 1 (2), 2785:J / Sigma FT-IR 1 (2), 146:C / Stains and Dyes Ref, 371 / Structure Index 1, 443:C:1 |
